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Figure 1 | BMC Complementary and Alternative Medicine

Figure 1

From: Black tea extract prevents lipopolysaccharide-induced NF-κB signaling and attenuates dextran sulfate sodium-induced experimental colitis

Figure 1

Effect of BTE on expression of LPS-induced pro-inflammatory mediators in BMM. RNA was isolated using TRIzol procedure, and 1 μg of total RNA was reverse transcribed and amplified using specific primer for IL-12p40, IL-23p19, IL-6, IL-1β and GAPDH. Band intensities were quantified by densitometry. Results are representative of 3 independent experiments. (A) The cells were pretreated with various concentrations of BTE (0-200 μg/ml) after which they were stimulated with 1 μg/ml of LPS for time indicated (1 h). BTE significantly reduced the LPS-induced IL-12p40, IL-23p19, IL-6, and IL-1β mRNA accumulations in various concentrations as determined by RT-PCR and densitometry. (B) The cells were pretreated with 100 μg/ml BTE for 1 h prior to different incubation times (1 or 4 h) with stimulation of 1 μg/ml LPS. BTE significantly reduced the LPS-induced IL-12p40, IL-23p19, IL-6, and IL-1β mRNA accumulations for different incubation times as determined by RT-PCR and densitometry.

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