Figure 6

WA down-regulates ERα expression at post-translational level by inducing proteasomal degradation of ERα protein. A, Western analysis of the expression of ERα after treatment with 100 μg/ml cychloheximide (Chx) in the absence or presence of 2.5 μM WA for 1, 2, 4, or 6 h. B, Western analysis of the expression of ERα after treatment with 30 μM MG132 (MG) for 1 h followed by 2.5 μM WA for 6 h. Proteins from both triton-soluble and triton-insoluble fractions were examined.