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Figure 6 | BMC Complementary and Alternative Medicine

Figure 6

From: ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Figure 6

Effect of RM on AMPK and glucose uptake. 3T3-L1 pre-adipocytes 2 days after confluence were induced to differentiate. (A) 3T3-L1 adipocytes were treated with RM (0, 50, 100 μg/ml) or insulin (100, 200 nM) for 30 minutes and the expression of phopho-AMPK was detected by Western blot analysis as described in Materials and Methods. Immunoblots are representative of 3 independent experiments. Data represents mean ± SD. *p < 0.05 vs adipocyte control cells. (B) Glucose uptake was determined in 3T3-L1 adipocytes treated with RM (0, 50, 100 μg/ml) or insulin (200 nM) as described in Materials and Methods. The results are mean ± SD (n = 12), *p < 0.05 vs adipocyte control cells.

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