Figure 5

Effect of RM on mRNA levels of perilipin and protein expression for perilipin. 3T3-L1 pre-adipocytes 2 days after confluence were induced to differentiate in the presence of RM (0-300 μg/ml). At day 9 adipocytes RNA and proteins were isolated. (A) mRNA levels of perilipin were determined by RT-PCR and (B) protein expression of perilipin was determined by Western blot analysis. Each experiment was repeated thrice and results are depicted as mean ± SD, statistical significance was calculated (letters that differ indicate, p ≤ 0.05).