Figure 1

Effect of RM on lipid accumulation during differentiation. 3T3-L1 pre-adipocytes 2 days after confluence were induced to differentiate in the presence of RM (0-300 μg/ml). At day 9 adipocytes were stained with Oil red O as shown in Figure 1A. (a) pre-adipocytes, (b) adipocytes [RM-0], (c) adipocytes [RM-100 μg/ml], (d) adipocytes [RM-200 μg/ml], (e) adipocytes [RM-300 μg/ml]. (B) Quantification of lipids was measured after eluting with isopropanol and reading the absorbance at 510 nm. (C) triglyceride and (D) glycerol concentration was determined as described in Materials and Methods. Each experiment was performed three times in triplicates. Results are depicted as mean ± SD, statistical significance was calculated (letters that differ indicate, p ≤ 0.05). For our studies we used cells up to passage twelve.