Figure 3
Zymography of Achilles tendon extracts. Each lane represents a sample of one animal. a, b- Gelatin zymography gel used to quantify activities of MMP-2 and MMP-9, respectively; c, d and e- Densitometry analysis of MMP-2, MMP-9 and total MMP-2, respectively. CS-control sedentary; HS- H. aphrodisiaca sedentary; CT- control trained; HT- H. aphrodisiaca trained. In the HT group the MMP-2 activity was reduced when compared with CS. MMPs activity was similar in both HS and CS groups, indicating the H. aphrodisiaca treatment did not inhibit MMP activity. MMP-9 latent (92 kDa), MMP-9 active (82 kDa), MMP-2 latent (72 kDa), MMP-2 intermediate (68 kDa), MMP-2 active (62 kDa). The columns are the mean ± SD. * Differences were significant for p < 0.05 (ANOVA) compared with CS group, by the Tukey test.