Figure 7

Effects of APS on apoptosis analyzed by JC1 assay. M-07e cells were treated as described above, incubated with JC-1 reagent and then subjected to flow cytometry analyses. (A). Dot plots of Normal, APS-treated, Control, Ly294002-treated, TPO-treated, and APS+Ly294002-treated samples. (B). Quantification and statistical analyses of treatment effects. Percentages of R1 (transitional cell subset containing both JC1 aggregates and monomers, representing early apoptotic cells), R2 (apoptotic cells containing mostly monomers, representing late apoptotic cells), R1+R2 (total apoptotic cells) cells are shown. Statistical tests were carried out between the control/treated samples and the corresponding normal samples respectively.*: p < 0.05; #: p < 0.01 and +: p < 0.001.