Skip to main content

Table 3 Antioxidant activity of protein fractions from UF and SFD cacao seeds

From: Antitumor activity against murine lymphoma L5178Y model of proteins from cacao (Theobroma cacao L.) seeds in relation with in vitro antioxidant activity

Sample

Unfermented seed

 

Semi-fermented dry seed

 
 

ABTS+ assay

 

ORAC assay

ABTS+ assay

 

ORAC assay

 

TEAC (mM Trolox/g protein)

IC50 (mg protein/mL)

TEAC (mM Trolox/g protein)

TEAC (mM Trolox/g protein)

IC50 (mg protein/mL)

TEAC (mM Trolox/g protein)

Albumin

0.14 ± 0.03 (3000)

3. 14 ± 0.08

0.44 ± 0.05 (70)

0.37 ± 0.01 (1500)*

1.51 ± 0.02*

0.65 ± 0.13 (50) *

Globulin

0.18 ± 0.03 (3000)

2.89 ± 0.08

0.17 ± 0.01 (300)

0.22 ± 0.04 (1500) *

2.21 ± 0.06*

0.29 ± 0.04 (150) *

Glutelin

0.88 ± 0.01 (600)**

0.58 ± 0.04**

1.21 ± 0.11 (70)**

1.32 ± 0.01 (300) *,**

0. 22 ± 0.02*,**

1.10 ± 0.73 (50) *,**

GHS

0.44 ± 0.11 (92)

0.081 ± 0.04

0.65 ± 0.44 (21)

0.44 ± 0.11 (92) *

0.081 ± 0.04*

0.65 ± 0.44 (21) *

  1. TEAC (Trolox equivalent antioxidant capacity), GHS (Reduced glutathione).
  2. Data are expressed as the mean of the results of two experiments realized by duplicate ± S.E.M.
  3. Values in parentheses indicate protein concentration assayed expressed as ppm
  4. * p < 0.05 unfermented seed compared with semifermented dry seed.
  5. ** p < 0.05 when is comparing seed protein fraction regardless cacao process.