Figure 3

Fractionation of semifermented-dry cacao protein by size exclusion chromatography (Albumin: Superdex G75 10/30 GL, equilibrium liquid and sample buffer 20 mM Tris-HCl, 150 mM NaCl, pH 7.2, flow rate 1 mL/min; globulin and glutelin: Superdex Hiload 200 16/60, equilibrium liquid and sample buffer 20 mM Tris-HCl, 150 mM NaCl, pH 7.2 and 20 mM potassium phosphate, 1.0 M NaCl, containing 1% (v/v) NLS, respectively, flow rate 1 mL/min).