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Table 1 Oligonucleotide primer sequences used in RT-PCR

From: Momordica charantia (bitter melon) inhibits primary human adipocyte differentiation by modulating adipogenic genes

Target Gene Primer sequence Cycling condition Size (bp)
PPAR γ2 Forward 5'-GCATGGTGCCTTCGCTGATGC-3' Reverse 5'-AGGCCTGTTGTAGAGCTGGGT-3' 95°C/5 min 95°C/1 min,55°C/1 min 72°C/1 min,72°C/7 min 4°C hold, 33 cycles 340
SREBP-1c Forward 5'-TTGCGCAAGGCCATCGACTACATT-3' Reverse 5'-ACAAGGGGCTGCTCTGGAAAGGTG-3' 94°C/5 min 94°C/1 min,55°C/1 min 72°C/1 min,72°C/7 min 4°C hold, 26 cycles 238
Perilipin Forward 5'-TGGAGACTGAGGAGAAGAAG-3' Reverse 5'-ATGTGAGAGGGGAGATGG-3' 94°C/1.5 min 94°C/45 sec,52°C/1.5 min 72°C/1 min,72°C/7 min 4°C hold, 23 cycles 120
Resistin Forward 5'-TGGTGGTGGTGGCTGTCCTGG-3' Reverse 5'-GAGTGAGATGTGGTCTGGGCG-3' 95°C/2 min 95°C/45 sec,55°C/1.5 min 72°C/45 sec,72°C/7 min 4°C hold, 39 cycles 145
GAPDH Forward 5'-AGTCAGCCGCATCTTCTTTTG-3' Reverse 5'-CTCCTGGAAGGGAGATGG-3' Condition adjusted as per the target gene 298