Target Gene | Primer sequence | Cycling condition | Size (bp) |
---|---|---|---|
PPAR γ2 | Forward 5'-GCATGGTGCCTTCGCTGATGC-3' Reverse 5'-AGGCCTGTTGTAGAGCTGGGT-3' | 95°C/5 min 95°C/1 min,55°C/1 min 72°C/1 min,72°C/7 min 4°C hold, 33 cycles | 340 |
SREBP-1c | Forward 5'-TTGCGCAAGGCCATCGACTACATT-3' Reverse 5'-ACAAGGGGCTGCTCTGGAAAGGTG-3' | 94°C/5 min 94°C/1 min,55°C/1 min 72°C/1 min,72°C/7 min 4°C hold, 26 cycles | 238 |
Perilipin | Forward 5'-TGGAGACTGAGGAGAAGAAG-3' Reverse 5'-ATGTGAGAGGGGAGATGG-3' | 94°C/1.5 min 94°C/45 sec,52°C/1.5 min 72°C/1 min,72°C/7 min 4°C hold, 23 cycles | 120 |
Resistin | Forward 5'-TGGTGGTGGTGGCTGTCCTGG-3' Reverse 5'-GAGTGAGATGTGGTCTGGGCG-3' | 95°C/2 min 95°C/45 sec,55°C/1.5 min 72°C/45 sec,72°C/7 min 4°C hold, 39 cycles | 145 |
GAPDH | Forward 5'-AGTCAGCCGCATCTTCTTTTG-3' Reverse 5'-CTCCTGGAAGGGAGATGG-3' | Condition adjusted as per the target gene | 298 |