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Table 1 Percentage yield, DPPH radical scavenging activity and total phenolic content of Blechnum orientale extracts

From: Blechnum Orientale Linn - a fern with potential as antioxidant, anticancer and antibacterial agent

Sample *Yield(g/100 g dry leaves) **DPPH IC50(μg/ml) **Total phenolic content (mg GAE/g dry weight)
Crude 17.2 ± 1.6 10.9 ± 1.6a 346 ± 48
PEf 6.3 ± 0.6 26.5 ± 2.2b 154 ± 10
Cf 0.1 ± 0.0 37.5 ± 3.0c 214 ± 14
EAf 1.6 ± 0.7 8.6 ± 0.5d 804 ± 72
Butf 2.7 ± 0.2 10.1 ± 1.1a 675 ± 62
Watf 6.5 ± 0.4 13.0 ± 1.3a 260 ± 9
Ascorbic acid   5.3 ± 0.1e  
Trolox-C   8.7 ± 0.1d  
α-Tocopherol   12.0 ± 0.7a  
BHT   17.2 ± 0.2f  
  1. *Percentage yield is expressed as mean ± SD from 3 batches of collection.
  2. ** Results are mean ± SD from 3 batches of fern collection and 3 sets of experiments conducted on each batch (n = 9).
  3. Cf = chloroform fraction; EAf = ethyl acetate fraction; Butf = butanol fraction; Watf = water fraction. Ascorbic acid, trolox-C, α-tocopherol and BHT (butylated hydroxytoluene) were used as standards.
  4. For each column, values followed by the same letters are not statistically different (P > 0.05).