Blechnum Orientale Linn - a fern with potential as antioxidant, anticancer and antibacterial agent

Table 1 Percentage yield, DPPH radical scavenging activity and total phenolic content of Blechnum orientale extracts

Sample	*Yield(g/100 g dry leaves)	**DPPH IC₅₀(μg/ml)	**Total phenolic content (mg GAE/g dry weight)
Crude	17.2 ± 1.6	10.9 ± 1.6^a	346 ± 48
PEf	6.3 ± 0.6	26.5 ± 2.2^b	154 ± 10
Cf	0.1 ± 0.0	37.5 ± 3.0^c	214 ± 14
EAf	1.6 ± 0.7	8.6 ± 0.5^d	804 ± 72
Butf	2.7 ± 0.2	10.1 ± 1.1^a	675 ± 62
Watf	6.5 ± 0.4	13.0 ± 1.3^a	260 ± 9
Ascorbic acid		5.3 ± 0.1^e
Trolox-C		8.7 ± 0.1^d
α-Tocopherol		12.0 ± 0.7^a
BHT		17.2 ± 0.2^f

*Percentage yield is expressed as mean ± SD from 3 batches of collection.
** Results are mean ± SD from 3 batches of fern collection and 3 sets of experiments conducted on each batch (n = 9).
Cf = chloroform fraction; EAf = ethyl acetate fraction; Butf = butanol fraction; Watf = water fraction. Ascorbic acid, trolox-C, α-tocopherol and BHT (butylated hydroxytoluene) were used as standards.
For each column, values followed by the same letters are not statistically different (P > 0.05).

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