Fig. 10

AST affects TGF-β-induced fibrosis in T-HECSs through the PI3K/AKT/NF-κB pathway. T-HESCs were incubated with TGF-β (10ng/ml) for 48 h prior to action with AST for 1 h (n = 3). (A) The expression and quantification of FN by western blot analysis. (B) The expression and quantification of p-NF-κB/NF-κB by western blot analysis. (C) The expression and quantification of p-AKT/AKT and BCL2 by western blot analysis. Analysis of data was done using One-way ANOVA or Dennett’s T3. AST indicates astilbin. *P < 0.05, **P < 0.01, ***P < 0.001. The original blots are presented in Additional File 10 Figure S1A-C