Trans-chalcone and quercetin down-regulate fatty acid synthase gene expression and reduce ergosterol content in the human pathogenic dermatophyte Trichophyton rubrum
© Bitencourt et al.; licensee BioMed Central Ltd. 2013
Received: 6 September 2012
Accepted: 4 September 2013
Published: 17 September 2013
Fatty acid synthase (FAS) is a promising antifungal target due to its marked structural differences between fungal and mammalian cells. The aim of this study was to evaluate the antifungal activity of flavonoids described in the scientific literature as FAS inhibitors (quercetin, trans-chalcone, ellagic acid, luteolin, galangin, and genistein) against the dermatophyte Trichophyton rubrum and their effects on fatty acid and ergosterol synthesis.
The antifungal activity of the natural products was tested by the microdilution assay for determination of the minimum inhibitory concentration (MIC). The effect of the compounds on the cell membrane was evaluated using a protoplast regeneration assay. Ergosterol content was quantified by spectrophotometry. Inhibition of FAS by flavonoids was evaluated by an enzymatic assay to determine IC50 values. Quantitative RT-PCR was used to measure transcription levels of the FAS1 and ERG6 genes involved in fatty acid and ergosterol biosynthesis, respectively, during exposure of T. rubrum to the flavonoids tested.
The flavonoids quercetin and trans-chalcone were effective against T. rubrum, with MICs of 125 and 7.5 μg/mL for the wild-type strain (MYA3108) and of 63 and 1.9 μg/mL for the ABC transporter mutant strain (ΔTruMDR2), respectively. The MICs of the fluconazole and cerulenin controls were 63 and 125 μg/mL for the wild-type strain and 30 and 15 μg/mL for the mutant strain, respectively. Quercetin and trans-chalcone also reduced ergosterol content in the two strains, indicating that interference with fatty acid and ergosterol synthesis caused cell membrane disruption. The MIC of quercetin reduced the number of regenerated protoplasts by 30.26% (wild-type strain) and by 91.66% (mutant strain). Half the MIC (0.5 MIC) of quercetin did not reduce the number of regenerated wild-type fungal colonies, but caused a 36.19% reduction in the number of mutant strain protoplasts. In contrast, the MIC and 0.5 MIC of trans-chalcone and cerulenin drastically reduced protoplast regeneration in the two strains. The FAS1 gene was repressed in the presence of MICs of quercetin, trans-chalcone, fluconazole and cerulenin. The ERG6 gene was induced in the presence of MICs of fluconazole and cerulenin and was repressed in the presence of MICs of trans-chalcone and quercetin. Trans-chalcone and quercetin inhibited the enzymatic activity of FAS, with IC50 values of 68.23 and 17.1 μg/mL, respectively.
Trans-chalcone and quercetin showed antifungal activity against T. rubrum, reducing ergosterol levels and modulating the expression of FAS1 and ERG6.
KeywordsAntifungal activity Antifungal agent Antifungal target Dermatophytes Trichophyton rubrum
Dermatophytosis is a cutaneous mycosis caused by fungi of the family Arthrodermataceae (Dermatophytes), which are able to digest keratin. This mycosis is a common infection worldwide . The main etiological agent of dermatophytosis is the anthropophilic and cosmopolitan fungus Trichophyton rubrum, which accounts for 69.5% of all dermatophytic infections [2, 3]. Infections caused by this species are difficult to treat and there is only a limited number of antifungal drugs available for clinical use, especially when compared to the arsenal of antibacterial drugs [4, 5].
Therefore, novel drugs with more specific and effective mechanisms of action against dermatophytes are urgently needed. In this respect, natural products provide a rich source of chemical diversity for the development of new drugs . However, the lack of suitable targets and the similarities between fungal and mammalian cells often result in compounds that are highly toxic to humans [7, 8]. An interesting target is fatty acid synthase (FAS), an enzyme that participates in endogenous fatty acid synthesis . The fact that this enzyme shows marked structural differences between fungal and mammalian cells makes it a promising target for the development of new antifungal drugs . The best known natural inhibitor of FAS is cerulenin, an epoxide produced by the fungus Cephalosporium caeruleus. This compound covalently binds to the catalytic site of FAS and disrupts the condensation reaction of acetyl-COA and malonyl-COA, inhibiting the biosynthesis of fatty acids and sterols in yeast .
In this study, we explored the antifungal activity of quercetin and five other flavonoids described as inhibitors of FAS against T. rubrum. DNA microarray studies using T. rubrum and synthetic inhibitors of FAS (PHS11A and PHS11B) have shown marked transcriptional modulation of several genes such as FAS1 and FAS2, ERG6 (a gene involved in ergosterol metabolism), and multidrug resistance genes [13, 14]. We evaluated the antifungal activity of flavonoids against T. rubrum and their effects on fatty acid and ergosterol synthesis using a wild-type strain and a mutant strain (ΔTruMDR2) that carries a disrupted version of an ABC transporter involved in multidrug resistance .
The T. rubrum strain H6 (ATCC MYA3108) and the mutant strain ΔTruMDR2 (obtained by disruption of the TruMDR2 gene of strain MYA3108) were submitted to standard techniques for fungal manipulation and growth as described previously by Fachin et al. .
Natural products and chemicals
Susceptibility of the MYA3108 and ΔTruMDR2 T. rubrum strains (1.0 to 8 × 105 CFU/mL) was tested by determining the minimum inhibitory concentration (MIC) of different concentrations of the flavonoids (0.475 to 1000 μg/mL) and controls (fluconazole and cerulenin: 1.9 to 1000 μg/mL) using the M38-A microdilution technique proposed by the Clinical and Laboratory Standards Institute (CLSI, 2002) and described by Fachin et al. . Microtiter trays were incubated at 28°C and MICs were recorded after 7 days of incubation. The MIC100 was defined as the lowest concentration of flavonoids that completely inhibited the growth of MYA3108 and ΔTruMDR2. The assays were carried out in three independent experiments performed in triplicate.
Quantification of ergosterol content
Mycelia of strain MYA3108 or ΔTruMDR2 were incubated in 50 mL liquid Sabouraud glucose medium for 24 h at 28°C under gentle shaking at 200 rpm. The mycelia were harvested aseptically and transferred to 20 mL fresh Sabouraud medium containing MICs of quercetin and of the controls cerulenin and fluconazole. The MIC and 0.5 MIC were used for trans-chalcone. Next, the material was incubated for 48 h under the same conditions as described above and harvested by filtration. Ergosterol was extracted as described by Arthington-Skaggs et al. (1999)  and quantified spectrophotometrically based on a standard curve of different concentrations of ergosterol (Sigma).
Protoplast regeneration assay
Protoplasts were obtained from each T. rubrum strain grown for 7 days in 30 mL lytic solution (20 mg/mL Trichoderma harzianum lysing enzymes purchased from Sigma-Aldrich; 0.7 M KCl and 1 M MgSO4, pH 6.8) by incubating the mycelium for approximately 4–6 h at 28°C under gentle agitation. The protoplasts were filtered through glass wool, collected by centrifugation (5 min, 4°C, 1556 g), and washed in 10 mL regeneration buffer (0.8 M NaCl, 10 mM CaCl2, 50 mM Tris–HCl, pH 7.5). The concentration of cells was adjusted to 1 × 104 protoplasts/mL. Regenerated protoplasts were selected on solid minimal medium  supplemented with 1 M sucrose, 0.2% casein and 0.07 mM NaNO3 by incubation with the MIC and 0.5 MIC concentrations of quercetin, trans-chalcone and cerulenin for 7 days at 28°C.
Extraction of total proteins for enzymatic assay
The method described by Li et al.  was used for the extraction of total proteins, with modifications. Total proteins were extracted from the yeast Saccharomyces cerevisiae using glass beads and 20 mg/mL of lysing enzymes (Sigma) in 125 mM phosphate buffer, pH 6.6, 1 mM EDTA, 1 mM DTT, 0.7 μg/mL pepstatin, 0.2 μg/mL aprotinin, and 0.2 μg/mL leupeptin. The lysate was centrifuged at 30,000 g for 30 min at 4°C and precipitated with 25% ammonium sulfate for 30 min under shaking on ice. The protein pellet was obtained by centrifugation at 12,000 g for 30 min at 4°C. The extracted proteins were resuspended in the same buffer and dialyzed for 24 h in phosphate buffer. Total proteins were quantified and then stored at 4-8°C for one week.
FAS enzymatic assay
The enzymatic assay was performed according to Li et al. , with modifications. Total proteins were quantified and diluted (500 mOD/min). The assay of FAS inhibition by flavonoids was carried out in 96-well plates in a final volume of 100 μL. First, the previously diluted proteins were incubated with quercetin and trans-chalcone for 30 min at room temperature. Next, 50 μL of this mixture was added to 250 mM reaction phosphate buffer containing 1 mM malonyl CoA, 1 mM NADPH, and 40 μM acetyl CoA. The microplate was read immediately for 10 min at 340 nm. The readings were obtained at intervals of 2 min until the end of analysis. FAS activity was calculated by subtracting the OD value obtained after 1 min from the OD value obtained after 10 min. The remaining activity was obtained in the same way and inhibition was calculated by comparison between control activity (considered to be 100%) and remaining activities of the treatments. The IC50 was determined by linear regression. The negative control consisted of protein plus reaction buffer. DMSO (1%) was also tested and did not interfere with FAS activity. Another control consisted of buffer reaction without protein.
Means were compared by the Scott-Knott test and a P value <0.05 was considered to indicate statistically significant differences.
MIC 100 (μg/mL) of the natural products against Trichophyton rubrum
Quantification of ergosterol content
Percent reduction of ergosterol content in the Trichophyton rubrum strains
MIC of quercetin
33.54 ± 8.95a
56.15 ± 4.06 a
MIC of cerulenin
40.72 ± 5.6a
49.48 ± 8.24 a
MIC of fluconazole
40.95 ± 9.05a
61.50 ± 6.73a
MIC of trans-chalcone
0.5 MIC of trans-chalcone
74.59 ± 5.35 c
77.85 ± 15.73 c
Protoplast regeneration assay
Percent reduction in the number of regenerated protoplasts in the Trichophyton rubrum strains
MIC of quercetin
30.26 ± 5.78 b
91.66 ± 8.33d
MIC of cerulenin
MIC of trans-chalcone
0.5 MIC of quercetin
36.19 ± 6.44a
0.5 MIC of cerulenin
65.26 ± 7.61b
0.5 MIC of trans-chalcone
83.24 ± 3.50c
Modulation of ERG6 and FAS1
FAS enzymatic assay
Fatty acid synthase inhibitory activity
Chalcones (1,3-diaryl-2-propen-1-one) are open-chain flavonoids that are mainly synthesized by plants. These compounds have been shown to exert significant antifungal activity, especially against dermatophytes . Most chalcones inhibit the biosynthesis of the yeast cell wall , but there is evidence that chalcones also block FAS in yeast (12) and fatty acid synthase II in mycobacteria .
In the present study, the MICs of quercetin, trans-chalcone, cerulenin and fluconazole were always lower for the mutant strain than for the wild-type strain (MYA3108). This higher susceptibility of the mutant strain might be explained by disruption of the TruMDR2 gene. This gene encodes an ABC transporter that is involved in the resistance to various antifungal agents, such as terbinafine, 4NQO, and ethidium bromide . To our knowledge, this is the first study reporting the involvement of the TruMDR2 transporter in flavonoid-mediated FAS inhibition.
A reduction of ergosterol content was observed in the two T. rubrum strains when the fungus was grown in the presence of the MIC of quercetin. However, a higher percent reduction of ergosterol content was found when the strains were grown in the presence of 0.5 MIC and MIC of trans-chalcone, demonstrating a relationship between fatty acid synthesis and ergosterol. In fungi, sterols are found in the plasma membrane where they play a role in membrane permeability.
The protoplast regeneration assay has been used to evaluate the level of plasma membrane damage caused by antifungal compounds [21, 22]. Quercetin and trans-chalcone reduced the number of regenerated protoplasts in the two strains, suggesting an effect of these compounds on the cell membrane. Interestingly, in the presence of the 0.5 MICs of cerulenin and trans-chalcone, protoplast regeneration was higher for the mutant strain grown in regeneration medium with a high sucrose concentration (1.5 M) when compared to the wild-type strain. On the other hand, ergosterol content was lower in the mutant strain (11.2 μg/mL) than in the wild-type strain (31.39 μg/mL) when the cells were cultured in Sabouraud medium (data not shown). In yeast, osmotic stress (NaCl and sorbitol) has been shown to increase the level of resistance of cells treated with drugs that inhibit ergosterol synthesis . This phenomenon observed for yeast may explain the better regeneration seen in the T. rubrum mutant strain. However, further studies are needed to clarify these results.
The expression of T. rubrum FAS1 (fatty acid synthases) and ERG 6 (ergosterol synthesis) genes is modulated by the synthetic FAS inhibitors PHS11A  and PHS11B . In this study, we also observed that the inhibition of fatty acid and ergosterol synthesis by quercetin and trans-chalcone may involve transcriptional modulation of these genes since they are down-regulated by both flavonoids. In addition to transcriptional modulation of the FAS1 gene, experiments using S. cerevisiae cells have shown that quercetin and trans-chalcone inhibit the enzymatic activity of FAS. Quercetin showed a better inhibitory activity, but trans-chalcone showed a better antifungal activity. This result suggests that the antifungal activity of trans-chalcone may be related to targets other than FAS, such as ergosterol synthesis. Ergosterol is a vital component of the fungal cell, but little is known about the genetics and biochemistry of the ergosterol biosynthesis pathway and only 20 genes involved in the biosynthesis of ergosterol in T. rubrum have been sequenced . Zhang et al.  demonstrated up-regulation of the FAS1, FAS2 and ERG6 genes in response to PHS11A. In addition, the ERG5, ERG6 and ERG25 genes have been shown to be up-regulated in Candida albicans strain SC5314-AR, which is resistant to fluconazole and amphotericin B. The higher expression of these genes may increase the conversion of lanosterol to eburicol and 14-methyl fecosterol. By altering the pathway at this particular point, the cell would no longer be susceptible to the effects of fluconazole or amphotericin B. Analysis of sterol content in this strain confirmed the hypothesis that resistance to the two antifungal agents was due to the accumulation of sterol intermediates, which is consistent with the inactivation of lanosterol demethylase, and to the increased expression of several ergosterol biosynthesis genes . According to Leber et al. , when ergosterol synthesis is inhibited, steryl esters are hydrolyzed and ergosterol levels remain sufficiently high to maintain membrane formation and growth for some time, and no genes involved in ergosterol synthesis are induced. Furthermore, ergosterol has been reported to regulate its own synthesis by negative feedback mechanisms [27–30].
The complete assessment of the mode of action of quercetin and trans-chalcone will require the analysis of additional genes and other assays that could demonstrate the interference of FAS inhibition with fatty acid homeostasis in T. rubrum. One possibility is supplementation of the growth medium with exogenous fatty acids in the presence of quercetin and trans-chalcone. Nevertheless, the present results indicate that these compounds can be explored for the development of new antifungal drugs.
Quercetin and trans-chalcone presented the best antifungal activity among the flavonoids tested. The results of the FAS enzymatic assay showed that quercetin is a better inhibitor of this enzyme than trans-chalcone. However, the lower MIC of trans-chalcone when compared to quercetin suggests the involvement of another cellular target in addition to FAS. Therefore, trans-chalcone is a potential candidate for the development of new antifungal drugs against T. rubrum since it simultaneously inhibits the synthesis of fatty acids and ergosterol, a fact reducing the risk of resistance. This property is another advantage since these two targets are specific of fungal cells.
This study was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (2009/12419-0; TT2 fellowship granted to B.G.M.), CNPq (PIBIC fellowship granted to T.T.K.), and CAPES through a fellowship granted to T.A.B. We thank the staff of the Biotechnology Unit, UNAERP, for general support, K. Markendorf for English revision, and Professor Nilce M. Martinez-Rossi for kindly providing the T. rubrum strains.
- Marconi VC, Kradin R, Marty FM, Hospenthal DR, Kotton CN: Disseminated dermatophytosis in a patient with hereditary hemochromatosis and hepatic cirrhosis: case report and review of the literature. Med Mycol. 2010, 48 (3): 518-527. 10.3109/13693780903213512.View ArticlePubMedGoogle Scholar
- Ameen M: Epidemiology of superficial fungal infections. Clinics in dermatology. 2010, 28 (2): 197-201.View ArticlePubMedGoogle Scholar
- Chen BK, Friedlander SF: Tinea capitis update: a continuing conflict with an old adversary. Curr Opin Pediatr. 2001, 13 (4): 331-335. 10.1097/00008480-200108000-00008.View ArticlePubMedGoogle Scholar
- Baddley JW, Moser SA: Emerging fungal resistance. Clin Lab Med. 2004, 24 (3): 721-735. 10.1016/j.cll.2004.05.003.View ArticlePubMedGoogle Scholar
- Projan SJ, Youngman PJ: Antimicrobials: new solutions badly needed. Curr Opin Microbiol. 2002, 5 (5): 463-465. 10.1016/S1369-5274(02)00364-8.View ArticlePubMedGoogle Scholar
- Roemer T, Xu D, Singh SB, Parish CA, Harris G, Wang H, Davies JE, Bills GF: Confronting the challenges of natural product-based antifungal discovery. Chem Biol. 2011, 18 (2): 148-164. 10.1016/j.chembiol.2011.01.009.View ArticlePubMedGoogle Scholar
- Monk BC, Cannon RD: Genomic pathways to antifungal discovery. Curr Drug Targets: Infect Disord. 2002, 2 (4): 309-329. 10.2174/1568005023342344.View ArticleGoogle Scholar
- Sturtevant J: Translation elongation-3-like factors: are they rational antifungal targets?. Expert Opin Ther Tar. 2002, 6 (5): 545-553. 10.1517/1472818.104.22.1685.View ArticleGoogle Scholar
- Leibundgut M, Maier T, Jenni S, Ban N: The multienzyme architecture of eukaryotic fatty acid synthases. Curr Opin Struct Biol. 2008, 18 (6): 714-725. 10.1016/j.sbi.2008.09.008.View ArticlePubMedGoogle Scholar
- Maier T, Leibundgut M, Boehringer D, Ban N: Structure and function of eukaryotic fatty acid synthases. Q Rev Biophys. 2010, 43 (3): 373-422. 10.1017/S0033583510000156.View ArticlePubMedGoogle Scholar
- Nomura S, Horiuchi T, Omura S, Hata T: he action mechanism of cerulenin. I. Effect of cerulenin on sterol and fatty acid biosynthesis in yeast. J Biochem. 1972, 71 (5): 783-796.PubMedGoogle Scholar
- Li XC, Joshi AS, ElSohly HN, Khan SI, Jacob MR, Zhang Z, Khan IA, Ferreira D, Walker LA, Broedel SE: Fatty acid synthase inhibitors from plants: isolation, structure elucidation, and SAR studies. J Nat Prod. 2002, 65 (12): 1909-1914. 10.1021/np020289t.View ArticlePubMedGoogle Scholar
- Zhang W, Yu L, Leng W, Wang X, Wang L, Deng X, Yang J, Liu T, Peng J, Wang J: cDNA microarray analysis of the expression profiles of Trichophyton rubrum in response to novel synthetic fatty acid synthase inhibitor PHS11A. Fungal genetics and biology: FG & B. 2007, 44 (12): 1252-1261. 10.1016/j.fgb.2007.03.002.View ArticleGoogle Scholar
- Yu L, Zhang W, Liu T, Wang X, Peng J, Li S, Jin Q: Global gene expression of Trichophyton rubrum in response to PH11B, a novel fatty acid synthase inhibitor. J Applied Microbiol. 2007, 103 (6): 2346-2352. 10.1111/j.1365-2672.2007.03521.x.View ArticleGoogle Scholar
- Fachin AL, Ferreira-Nozawa MS, Maccheroni W, Martinez-Rossi NM: Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum. J Med Microbiol. 2006, 55 (Pt 8): 1093-1099.View ArticlePubMedGoogle Scholar
- Arthington-Skaggs BA, Jradi H, Desai T, Morrison CJ: Quantitation of ergosterol content: novel method for determination of fluconazole susceptibility of Candida albicans. J Clin Microbiol. 1999, 37 (10): 3332-3337.PubMedPubMed CentralGoogle Scholar
- Cove DJ: The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et biophysica acta. 1966, 113 (1): 51-56. 10.1016/S0926-6593(66)80120-0.View ArticlePubMedGoogle Scholar
- Nowakowska Z: A review of anti-infective and anti-inflammatory chalcones. Eur J Med Chem. 2007, 42 (2): 125-137. 10.1016/j.ejmech.2006.09.019.View ArticlePubMedGoogle Scholar
- Boeck P, Leal PC, Yunes RA, Filho VC, Lopez S, Sortino M, Escalante A, Furlan RL, Zacchino S: Antifungal activity and studies on mode of action of novel xanthoxyline-derived chalcones. Arch Pharm. 2005, 338 (2–3): 87-95.View ArticleGoogle Scholar
- Brown AK, Papaemmanouil A, Bhowruth V, Bhatt A, Dover LG, Besra GS: Flavonoid inhibitors as novel antimycobacterial agents targeting Rv0636, a putative dehydratase enzyme involved in Mycobacterium tuberculosis fatty acid synthase II. Microbiology. 2007, 153 (Pt 10): 3314-3322.View ArticlePubMedGoogle Scholar
- Jung HJ, Park Y, Sung WS, Suh BK, Lee J, Hahm K-S, Lee DG: Fungicidal effect of pleurocidin by membrane-active mechanism and design of enantiomeric analogue for proteolytic resistance. Biochimica et Biophysica Acta (BBA) - Biomembranes. 2007, 1768 (6): 1400-1405. 10.1016/j.bbamem.2007.02.024.View ArticleGoogle Scholar
- Lee DG, Park Y, Kim HN, Kim HK, Kim PI, Choi BH, Hahm K-S: Antifungal Mechanism of an Antimicrobial Peptide, HP (2–20), Derived from N-Terminus of Helicobacter pylori Ribosomal Protein L1 against Candida albicans. Biochem Biophys Res Communications. 2002, 291 (4): 1006-1013. 10.1006/bbrc.2002.6548.View ArticleGoogle Scholar
- Young LY, Hull CM, Heitman J: Disruption of ergosterol biosynthesis confers resistance to amphotericin B in Candida lusitaniae. Antimicrobial agents and chemotherapy. 2003, 47 (9): 2717-2724. 10.1128/AAC.47.9.2717-2724.2003.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang W, Yu L, Yang J, Wang L, Peng J, Jin Q: Transcriptional profiles of response to terbinafine in Trichophyton rubrum. Appl Microbiol Biotechnol. 2009, 82 (6): 1123-1130. 10.1007/s00253-009-1908-9.View ArticlePubMedGoogle Scholar
- Barker KS, Crisp S, Wiederhold N, Lewis RE, Bareither B, Eckstein J, Barbuch R, Bard M, Rogers PD: Genome-wide expression profiling reveals genes associated with amphotericin B and fluconazole resistance in experimentally induced antifungal resistant isolates of Candida albicans. J Antimicrob Chemother. 2004, 54 (2): 376-385. 10.1093/jac/dkh336.View ArticlePubMedGoogle Scholar
- Leber R, Zinser E, Hrastnik C, Paltauf F, Daum G: Export of steryl esters from lipid particles and release of free sterols in the yeast, Saccharomyces cerevisiae. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1995, 1234 (1): 119-126. 10.1016/0005-2736(94)00270-Y.View ArticleGoogle Scholar
- Pinto WJ, Lozano R, Nes WR: Inhibition of sterol biosynthesis by ergosterol and cholesterol in Saccharomyces cerevisiae. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1985, 836 (1): 89-95. 10.1016/0005-2760(85)90224-3.View ArticleGoogle Scholar
- Servouse M, Karst F: Regulation of early enzymes of ergosterol biosynthesis in Saccharomyces cerevisiae. Biochem J. 1986, 240 (2): 541-547.View ArticlePubMedPubMed CentralGoogle Scholar
- Casey WM, Keesler GA, Parks LW: Regulation of partitioned sterol biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1992, 174 (22): 7283-7288.PubMedPubMed CentralGoogle Scholar
- Smith SJ, Crowley JH, Parks LW: Transcriptional regulation by ergosterol in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1996, 16 (10): 5427-5432.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/13/229/prepub
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